Chromatography
Chromatography Units
GE Healthcare AKTA Prime Plus
BLE1800511
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ÄKTAprime plus is a lab-scale chromatography system that performs simple purifications of tagged and untagged proteins as well as antibodies.
- Optimal results with HiTrap, HiPrep and HiLoad prepacked columns.
- Specific and quick methods for tagged protein purification.
- Simple-to-use wirh preprogrammed templates covering all common techniques.
ÄKTAprime Plus is Compact and FlexibleÄKTAprime plus is a compact one-step lab-scale protein purification system. For reliability and convenience, we recommend HiTrap, HiPrep, or HiLoad columns that simply snap on to the side of the unit. The system has preprogrammed methods for typical applications such as purification of affinity tagged proteins, MAbs and sample clean-up. ÄKTAprime plus is a flexible system that can be used for more general purification tasks such as buffer exchange, gel filtration, ion exchange, HIC, and affinity chromatography.
ÄKTAprime plus includes a system pump, fraction collector, and monitors for UV, conductivity, and pH (optional). Valves for buffer selection, sample injection, gradient formation, and flow diversion are also integrated into the system.
ÄKTAprime plus Complete Pack size1 Piece
Order Information Excluding recorder
Application Simple purification of tagged and untagged proteins
Flow Rate 0.1 ml/min-50 ml/min
Operating Pressure Max.1 MPa (145 psi)
Operating Pressure [Sample Pump] Max.N/A (N/A)
Tubing i.d. [Flow Path] 0.75 mm
Single Wavelength Detection Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm. Optional zink lamp with 214 nm filter.
Multiple Wavelength Detection No
Flow Cell Path Length 2 mm (optional 5 mm)
Multiple Sample Injection Optional, up to 2 samples
Autosampler Injection No
Automatic Buffer Preparation No
Buffer Selection2 buffer inlets
Multi-Step Purification Optional, up to 2 steps
Relative Humidity 20%-95% (non condensing)
pH Stability Long Term 2-12
Viscositymax 10 cP (<10 ml/min), max 5 cP (>10 ml/min)
Width 400 mm
Height 530 mm
Depth 450 mm
Weight 13 kg
Voltage100-120/220-240 VAC
Power Consumption 90 VA
Protection Class IP 20 (Housing)
IP 44 (Flow cell)
Compliance The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended.
Fully Refurbished GE Healthcare Akta Explorer FPLC System
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BLE1800515
Application Purification of microgram to gram quantities of protein
Flow Rate 0.01-100 ml/min
Operating Pressure Max.10 M Pa (1450 psi)
Operating Pressure [Sample Pump] Max.10 M Pa (290 psi)
Tubing i.d. [Flow Path] 0.75 mm
Multiple Wavelength Detection Yes, up to 3 wavelengths
UV Wave length190-700 nm
Flow Cell Path Length 2 mm (optional 10 mm)
pH Monitoring 2-12
Multiple Sample Injection Yes, up to 8 samples
Autosampler Injection Optional
Automatic Buffer Preparation BufferPrep
Buffer Selection 11 buffer inlets
Relative Humidity 10%-95% (non condensing)
pH Stability Long Term 1-13
pH Stability Short Term 1-14
Viscosity max 5 cP
Width 480 mm
Height 610 mm
Depth 470 mm
Weight 67 kg
Voltage100-120/220-240 VAC
Power Consumption 600 VA
Protection Class IP 20
Pump Type Piston pump, metering type
Safety Certifications CE 89/336/EEC (EMC directive), CE 73/23/EEC (LV directive), EN-61010-1 (IEC1010-1, UL3101-1, CSA22.2 1010-1)
Compliance The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.
GE Healthcare Akta Flux 6 Flow Filtration System
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BLE1800513
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ÄKTA flux is a semi-automated tangential flow filtration/cross flow filtration system for concentration and diafiltration as well as cell harvest and clarification.
- Low working and hold-up volumes to support a wide range of concentration factors.
- Flexible handling of both hollow fiber filter cartridges and cassette filters.
- Versatile design enables use in both ultrafiltration and microfiltration applications.
- Available in two versions: ÄKTA flux s for research and filter screenings, and ÄKTA flux 6 for process development and scale-up experiments.
- For automated single-use filtration, GE offers ÄKTA readyflux.
Filtration made easy
Semi-automated features enable end-point control, such as constant retentate volume (CRV), and data logging, and frees time for other tasks in the lab. Process information and control are conveniently monitored and handled from the easy-to-use touchscreen.
Handles low working volumes
The ÄKTA flux system can handle low working and hold-up volumes to support a wide range of concentration factors. The ÄKTA flux filtration system is well-suited for use in protein purification workflows as a complement to the ÄKTA chromatography systems.
Application: Semiautomated crossflow filtration system
Recirculation Volume Min.: 108.5 mL
Operating Pressure Max.: 4 bar
Width: 640 mm
Length: 520 mm
Height: 720 mm
Weight: 53 kg
Connections: ½" TC
Hold-Up Volume Recirculation Line: 78.5 mL
Mixing Principle: Magnetic Stirbar
Material Compliance: USP VI, CFR 177, animal free origin (AFO) or complying with EMEA/410/01.
Main Voltage: 100-120/220-240 V AC, + 10%
Power Consumption: Max 400 VA
Protection Class: IP 21
Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare.
Permeate Flow Rate Min.: 20 mL/min
Permeate Flow Rate Max.: 1000 mL/min
Transfer Flow Rate Min.: 20 mL/min
Transfer Flow Rate Max.: 1000 mL/min
Wetted Part: Yes
Material: Polypropylene, Silicone, EPDM, PTFE, PVDF, Tygon
Thermo Fisher Easy NLC-1000
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BLE1800441
The Thermo Scientific EASY-nLC 1000 is a split-free, nano-flow liquid chromatograph optimized for separating biomolecules such as proteins and peptides at ultra high pressures up to 1000 bar or 15,000 psi. The instrument seamlessly integrates with various mass spectrometers, securing instant trouble-free operation. EASY-nLC 1000 is a fully integrated LC-system with a binary nanoflow gradient pump, a cooled autosampler, switching valves and high precision flow sensors for accurate solvent control before high-pressure mixing. The market leading operating pressure of the EASY-nLC 1000™ system offers an unprecedented choice of columns and is designed to help researchers achieve top performance in LC-MS
Amersham Pharmacia Biotech BPG 8 Liter Portable Glass Column for Sale
Contact to purchaseBLE160582/BLE1700379/BLE1900343
BPG columns are glass chromatography columns designed to meet the needs of process development and biopharmaceutical manufacture; hygienic design and operation; easy, efficient packing and running with the single-screw adapter.
- Scalable from 100 mm to 450 mm.
- Pressure rating from 2.5 Bar to 8 Bar, depending on column size.
- Glass column tube allows efficient and reproducible packing.
- Material Certificates Reports (BPG MCR) available for ordering.
- Installation and Operational Qualification Documentation Packages available.
- Hygienic design and operation. Microbial attachment and growth is prevented through the use of calibrated precision glass, high-grade electropolished stainless steel and an absence of dead pockets.
- Easy, efficient packing and running with the single-screw adapter.
- All polymeric materials meet the requirements for USP class VI, described in USP <88> Biological Reactivity Tests, in vivo
BPG columns are glass columns designed for use in the production of biopharmaceuticals or any product made in a regulated or current Good Manufacturing Practice (cGMP) environment. The columns are manufactured with materials carefully selected for their compatibility with the solvents most commonly used in biopharmaceutical manufacture. All polymeric materials are approved according to USP Class VI tests for toxicity.
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Amersham Pharmacia Biotech 3.5 Liter Chromatography Column (Model 56-0251-63)
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BLE1900240
Amersham Pharmacia BPG Chromatography Column 3.5L 8 Bar # 56-0251-63
GE Healthcare BPG 450-500 Chromatography Column Glass
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BLE1900227/BLE1900228
GLASS ONLY!
GE Healthcare BPG 300-500 Portable Glass Chromatography Column
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BLE1900272
Amersham Pharmacia BPG 15 Liter Chromatography Column
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BLE1900505
Millipore Quickscale 38 Liter Chromatography Column (Cat No GA301511)
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BLE1900503
Millipore Quikscale 2 Liter Chromatography Column GAG 70x550 (Cat No GA072711) for Sale
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Never Been Used - In Original Box
BLE1700382
Millipore Quikscale 2 Liter Chromatography
Column Vessel Number GAG 70x550 7 bar
Column Assembly. QS70 . YR:2007
Max Pressure: 7 Bar operating temp: 2-30C
QS70 stand for "Quikscale" 70x550
bed volume 0.04–2.08 L
Product Information
Connectors 3/4 in. Sanitary Clamp (1 in. diameter flange)
Maximum Differential Pressure, bar (psid) 7 bar (100 psid)
Maximum Inlet Pressure, bar (psi) 6.9 bar (100 psi)
Operating Temperature Range 2 to 30 °C
Applications
Key Applications Protein Purification
Physicochemical Information
Bed Volume 0.04–2.08 L
Dimensions
Bed Height 10–540 mm
Cross-sectional Area 38.5 cm²
Height 550 mm
Length 55.1 cm (21.7 in.)
Diameter 7.0 cm (2.75 in.)
Materials Information
Bed Support Material Stainless Steel 1.4539
Column Tube Material Borosilicate Glass
External Surface Material
Glass
Polypropylene
316L stainless steel
Flow Cell Material
Polypropylene
316L stainless steel
Seal Material EPDM
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