Wyatt Technologies Dawn Helios II (Model WH-02)
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BLE2000163
The DAWN® HELEOS® II is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. The DAWN offers the highest sensitivity, the widest range of molecular weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.
Measurements
Molar Mass Range 200 Da to 1000 MDa1
Molecular Size Range (rg)≈ 10 to 500 nm1, up to 1000 nm with shape-specific models
Molecular Size Range (Rh,with WyattQELS DLS module or DynaPro NanoStar) Depends on the scattering angle:
In flow mode, 0.5 nm – 300 nm1,2
batch mode, 0.5 nm – 1 µm1
Sensitivity 10 ng of 100 kDa polystyrene in THF3
200 ng of BSA in PBS4
Fluidics
Mobile Phase Compatibility All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.
Optics
Detectors
MALS Detectors High-gain, high dynamic range, static light scattering detectors at 18 angles.
Auxiliary Detectors Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.
A/D Resolution 24 bit (detector dynamic range > 16,000,000) -
WyattQELS dynamic light scattering module installs directly inside the DAWN chassis.
DLS Detector (optional)
Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument may be installed in the DAWN.Laser Properties Laser Wavelength658 nm; 785 nm available for fluorescence reduction (with lower sensitivity than 658 nm) Laser Power ControlProgrammable 10% - 100%Flow CellsFused Silica, optimal for solvent refractive index less than 1.50
F2, optimal for solvent refractive index above 1.50Temperature OptionsAmbient
Heated/Cooled (HC) -15°C to +150°C
Ultra-High-Temperature (UHT) ambient to +210°CElectronics Analog Inputs4 differential analog inputs with 24 bit resolution. Input range -10 V to +10 V Analog Outputs2 analog outputs from user settable measurements channels -10 V to +10 V Other Inputs/OutputsAlarm in, Alarm retransmit, Auto-Inject in, Auto inject contact closure, Recycle in, Recycle valve power Computer Interface Ethernet Transmission Rate36.6 to 0.0001 Hz Front Panel Display162.5 mm, 16-bit, high resolution touch screen displays signal graphs, instrument settings and diagnosticsDimensions60 cm (L) x 36 cm (W) x 23 cm (H)
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