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  • Wyatt Technologies Dawn Helios II (Model WH-02)

Wyatt Technologies Dawn Helios II (Model WH-02)

SKU: BLE2000163
$3,700.00
$3,700.00
Unavailable
per item

Free shipping available

BLE2000163


The DAWN® HELEOS® II is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. The DAWN offers the highest sensitivity, the widest range of molecular weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.

The DAWN is most often used in conjunction with Gel Permeation Chromatography (GPC-MALS) or Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.



Measurements    

Molar Mass Range 200 Da to 1000 MDa1   

Molecular Size Range (rg)≈ 10 to 500 nm1, up to 1000 nm with shape-specific models   

Molecular Size Range (Rh,with WyattQELS DLS module or DynaPro NanoStar) Depends on the scattering angle:

In flow mode, 0.5 nm – 300 nm1,2

batch mode, 0.5 nm – 1 µm1   

Sensitivity 10 ng of 100 kDa polystyrene in THF3

200 ng of BSA in PBS4

Fluidics    

Mobile Phase Compatibility All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.

Optics 

Detectors    

MALS Detectors High-gain, high dynamic range, static light scattering detectors at 18 angles.   

Auxiliary Detectors Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.   

A/D Resolution 24 bit (detector dynamic range > 16,000,000) -

WyattQELS dynamic light scattering module installs directly inside the DAWN chassis.

DLS Detector (optional)


Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument may be installed in the DAWN.Laser Properties    Laser Wavelength658 nm; 785 nm available for fluorescence reduction (with lower sensitivity than 658 nm)   Laser Power ControlProgrammable 10% - 100%Flow CellsFused Silica, optimal for solvent refractive index less than 1.50

F2, optimal for solvent refractive index above 1.50Temperature OptionsAmbient

Heated/Cooled (HC) -15°C to +150°C

Ultra-High-Temperature (UHT) ambient to +210°CElectronics    Analog Inputs4 differential analog inputs with 24 bit resolution. Input range -10 V to +10 V   Analog Outputs2 analog outputs from user settable measurements channels -10 V to +10 V   Other Inputs/OutputsAlarm in, Alarm retransmit, Auto-Inject in, Auto inject contact closure, Recycle in, Recycle valve power   Computer Interface Ethernet   Transmission Rate36.6 to 0.0001 Hz   Front Panel Display162.5 mm, 16-bit, high resolution touch screen displays signal graphs, instrument settings and diagnosticsDimensions60 cm (L) x 36 cm (W) x 23 cm (H)


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