Molecular Devices Spectramax Gemini XS Monochromatic
Fluorescence including FI, TRF and Luminescence
Equipped with UV-VIS Fluorescence
The Gemini XS spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.
Instrument Description and Capabilities:
Applications
Features
SoftMax Pro PC/MAC software
SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.
Engineered for superior performance and reliability
Gemini dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings. Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.
Specifications**
Fluorescence photometric performance
Dual Monochromators - 1 nm increment selection
EX Wavelength - 250-850 nm
EM Wavelength - 360-850 nm
Bandwidth (Excitation & Emission) - 9 nm
Detection Limit (Signal 3X SD of Baseline) - 2.0 fmol/well FITC 200 µL in 96 wells
Time Resolved Fluorescence (secondary mode)
Data Collection 50-1450 µsec, 200 µsec increments
Sensitivity 0.5 fmol/well
Luminescence Photometric Performance
Wavelength Range 250-850nm
Detection Limit 10 amol/well Alkaline Phosphatase 200 µl/well
General photometric specifications
Microplate Formats 6-, 12-, 24-, 48-, 96-, and 384-well
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time*** 96 wells in <27 seconds 384 wells in <45 seconds
Shaker Time 0 to 999 seconds
Temperature Control (Chamber) Ambient + 4 °C, to 45 °C
Fluorescence including FI, TRF and Luminescence
Equipped with UV-VIS Fluorescence
The Gemini XS spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.
Instrument Description and Capabilities:
- Fluorescence
- Time Resolved Fluorescence TRF
- Fluorescence Intensity top and bottom capabilities FI
- Fluorescence filters included in 1nm range
- Temperature control and microplate-shaking included
- Adjustable bandwidth range
- Luminescence glow reading mode
- 6-384-well plate capability
Applications
- Cellular and molecular binding studies
- Nucleic acid quantitation
- Cytoxicity assays
- SNP genotype detection and molecular beacons
- Enzyme activity
- CatchPoint® cAMP Assay Kit
Features
- Dual Monochromators — The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
- Wavelength Scanning — The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
- Well Scanning — Gemini XS can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.
- Auto PMT Gain — Because a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini XS features “Auto PMT Gain” to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.
SoftMax Pro PC/MAC software
SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.
Engineered for superior performance and reliability
Gemini dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings. Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.
Specifications**
Fluorescence photometric performance
Dual Monochromators - 1 nm increment selection
EX Wavelength - 250-850 nm
EM Wavelength - 360-850 nm
Bandwidth (Excitation & Emission) - 9 nm
Detection Limit (Signal 3X SD of Baseline) - 2.0 fmol/well FITC 200 µL in 96 wells
Time Resolved Fluorescence (secondary mode)
Data Collection 50-1450 µsec, 200 µsec increments
Sensitivity 0.5 fmol/well
Luminescence Photometric Performance
Wavelength Range 250-850nm
Detection Limit 10 amol/well Alkaline Phosphatase 200 µl/well
General photometric specifications
Microplate Formats 6-, 12-, 24-, 48-, 96-, and 384-well
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time*** 96 wells in <27 seconds 384 wells in <45 seconds
Shaker Time 0 to 999 seconds
Temperature Control (Chamber) Ambient + 4 °C, to 45 °C