Zeiss Apotome 1144-700 Structured Illumination Microscopy
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Fluorescence excitation light passes through two glass plates in the ApoTome slider. The grid pattern found on the first glass plate is imprinted in the excitation light. The second glass plate is tilted by a scanning mechanism, thereby slightly moving the beam path with the imprinted grid pattern up and down. The excitation light (green) with the grid pattern is then projected onto the specimen via a conventional fluorescence filter set. The emission light (red) is gathered by the objective and imaged to the camera sensor.
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