Miltenyi MACSQuant 10 Flow Cytometer
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BLE2000414
Laser excitationSpatially separated:
405 nm, 40 mW
488 nm, 30 mW
640 nm, 20 mW Emission detectorsFSC: 488/10 nm
SSC: 488/10 nm
V1: 450/50 nm
V2: 525/50 nm
B1: 525/50 nm
B2: 585/40 nm
B3: 655–730 nm
B4: 750 nm LP
R1: 655–730 nm
R2: 750 nm LPFluorescence sensitivity and resolutionMESFs (CV <5%):
FITC <200
PE <100
APC <150Flow cell dimensions200×250 μmFluorescence detectorsOptimized with spectrally matched PMTs for all channels Fluidics
Minimal uptake volume11 μL (25 μL recommended for volumetric counting applications)Sample flow rate25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second Measurement speed2,3<25 minutes per 96-well plate (5 μL measurement volume per well)Sample uptake1–450 µLMaximal event rateUp to 15,000 events/secondTechnical specifications
Footprint385.5×284.5 mm (15.18×11.2 in)Size (width×depth)
Height (adjustable touchscreen)669×400 mm (26.34×15.75 in)
394–553.5 mm (15.51–21.79 in)Size with miniSampler Plus (width×depth)669×500 mm (26.34×19.69 in) Weight50 kg (110 lbs)Monitor15.6" LCD touchscreenPower requirements100–240 V~, 50/60 HzPower consumption (standard use)450 WRAM8 GB DDR4 (SO-DIMM)Mass storage500 GB SSDPorts4× USB 2.0 ports, 6× USB 3.0 ports (2 at display),
2× DisplayPort, 2× LAN, DVI, RE-232, AudioEmission sound pressure level at workstation<61 dB(A)Performance
Sample carry-over2,40.01%Fluorescence performance5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scalesData management
Operating systemEmbedded operating systemMeasurement parametersArea, width, height for all parameters, with time and volumeSignal processing>18 bit dynamic range in area with 32 bit floating point signal processingCompensationAutomated, or manual with 8×8 matrix, during or after acquisitionData files.mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)